| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Braun, Gretchen L (9)
|
ADNTS |
2:57.69 |
2:50.39 |
-7.30 |
| 2 |
Stoup, Anna L (9)
|
ADCPP |
3:07.54 |
3:03.06 |
-4.48 |
| 3 |
Zhang, Stella Y (9)
|
ADCPP |
3:02.14 |
3:04.44 |
2.30 |
| 4 |
Saunders, Molly A (9)
|
ADHAWK |
3:34.18 |
3:22.64 |
-11.54 |
| 5 |
Hong, Allison (9)
|
ADCPP |
3:17.75 |
3:24.47 |
6.72 |
| 6 |
Carsillo, Ava C (7)
|
ADDELM |
|
3:25.69 |
- |
| 7 |
DeLair, Charlize J (9)
|
ADUSAM |
|
3:30.10 |
- |
| 8 |
Sellnow, Emma M (9)
|
ADEELS |
3:41.23 |
3:39.90 |
-1.33 |
| 9 |
Halverson, Zooey M (9)
|
ADUN |
3:47.44 |
3:41.26 |
-6.18 |
| 10 |
Agro, Lindsay M (9)
|
ADUSAM |
|
3:43.10 |
- |
| 11 |
Yafayeva, Roxana Z (8)
|
ADDELM |
|
3:56.58 |
- |
|
Pedersen, Alexandra J (9)
|
ADBENN |
|
DQ |
- |
|
Cronlund, Miranda M (8)
|
ADDELM |
|
DQ |
- |
|
Sanderson, Skyler G (9)
|
ADNTS |
3:35.00 |
DQ |
- |
|
Kline, Keaghan E (8)
|
NESNAP |
|
DQ |
- |
|
Smith, Lena T (8)
|
NESNAP |
|
DQ |
- |